Direct Molecular Identification of the Mouse Pink-Eyed Unstable Mutation by Genome Scanning

• DOI: 10.1126/science.1673574
• Publication Date: 2006-10-05T22:10:16Z
• Authors: Murray H. Brilliant; Yoichi Gondo; Eva M. Eicher
• Keywords: Southern blot; Reversion; genomic DNA

DNA sequences associated with the mouse pink-eyed unstable mutation were identified in absence of closely linked molecular markers and without prior knowledge encoded gene product. This was accomplished by "genome scanning," a technique which high-resolution Southern blots genomic DNAs hybridized to dispersed moderately repetitive sequence. In this assay, distinguished from wild-type revertant mice enhanced hybridization one several hundred resolved fragments. The fragment showing cloned found lie within duplication that is located close to, or within, dilution locus. may mediate high reversion frequency characteristic mutation.