A Fluorescent Modification of Adenosine Triphosphate with Activity in Enzyme Systems: 1, N 6 -Ethenoadenosine Triphosphate

• DOI: 10.1126/science.175.4022.646
• Publication Date: 2006-10-05T14:03:38Z
• Authors: John A. Secrist; Jorge R. Barrio; Nelson J. Leonard
• Keywords: Adenosine Diphosphate; 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Adenosine Triphosphate; Hexokinase; Muscles; Phosphofructokinase-1; Spectrum Analysis; Yeasts; Pyruvate Kinase; Imidazoles; Fluorescence

A new, highly fluorescent adenosine triphophate (ATP) analog, 1, N 6 -ethenoadenosine triphosphate, has been synthesized. Its fluorescence properties, including the long fluorescence lifetime and the possibility of detection at very low concentrations, in conjunction with its activity in the representative enzyme systems here reported, make it a valuable probe of enzymic mechanism and structure.