Recognition by Human V γ 9/V δ 2 T Cells of a GroEL Homolog on Daudi Burkitt's Lymphoma Cells

• DOI: 10.1126/science.1978758
• Publication Date: 2006-10-05T22:10:14Z
• Authors: Paul Fisch; Miroslav Malkovsky; Susan Kovats; Els Sturm; Erik Braakman; Bruce S. Klein; Stephan D. Voss; Lawrence W. Morri...; Robert DeMars; William J. Welch; Reinder L. H. Bol...; Paul M. Sondel
• Keywords: 0301 basic medicine; Antigens, Bacterial; Immunoglobulin delta-Chains; Immunoglobulin gamma-Chains; T-Lymphocytes; Histocompatibility Antigens Class I; Immunoglobulin Variable Region; Receptors, Antigen, T-Cell; Antigen-Presenting Cells; Gene Expression; Chaperonin 60; Burkitt Lymphoma; Clone Cells; Mycobacterium; 3. Good health; 03 medical and health sciences; Bacterial Proteins; Antigens, Neoplasm; Escherichia coli; Tumor Cells, Cultured; Humans; Heat-Shock Proteins; Immunosorbent Techniques

All human γδ T cells coexpressing the products of the variable (V) region T cell receptor (TCR) gene segments V γ 9 and V δ 2 recognize antigens from some mycobacterial extracts and Daudi cells. Exogenous and endogenous ligands on the cell surface, homologous to the groEL heat shock family, induced reactivities that resembled superantigen responses in this major subset of human peripheral blood γδ T cells. Stimulation of human V γ 9/V δ 2 T cells is not restricted by human leukocyte antigens (HLA), including nonpolymorphic β 2 -microglobulin (β 2 M)-associated class Ib molecules. These data may be important for understanding the role of γδ T cells in autoimmunity and in responses to microorganisms and tumors.