Induction by Antigen of Intrathymic Apoptosis of CD4 + CD8 + TCR lo Thymocytes in Vivo

• DOI: 10.1126/science.2125367
• Publication Date: 2006-10-05T22:10:14Z
• Authors: Kenneth M. Murphy; Amy B. Heimberger; Dennis Y. Loh
• Keywords: Antigens, Differentiation, T-Lymphocyte; 0301 basic medicine; Ovalbumin; CD8 Antigens; T-Lymphocytes; Molecular Sequence Data; Receptors, Antigen, T-Cell; Mice, Transgenic; Thymus Gland; Mice; Microscopy, Electron; 03 medical and health sciences; Phenotype; Phagocytosis; CD4 Antigens; Animals; Amino Acid Sequence

In order to examine the mechanisms by which clonal deletion of autoreactive T cells occurs, a peptide antigen was used induce antigen-reactive thymocytes in vivo. Mice transgenic for cell receptor (TCR) that reacts this contain progress from immature mature phenotype. Intraperitoneal administration mice results rapid CD4 + CD8 TCR lo thymocytes. Apoptosis cortical can be seen within 20 hours treatment. These provide direct evidence vivo role apoptosis development antigen-induced tolerance.