Cloning and Expression of a Developmentally Regulated Protein That Induces Mitogenic and Neurite Outgrowth Activity

• DOI: 10.1126/science.2270483
• Publication Date: 2006-10-05T22:10:14Z
• Authors: Yue-Sheng Li; Peter G. Milner; Anil K. Chauhan; Mark A. Watson; Ruth M. Hoffman; Charles M. Kodner; Jeffrey Milbrandt; Thomas F Deuel
• Keywords: Pleiotrophin; Neurite; Cloning (programming)

A heparin binding mitogenic protein isolated from bovine uterus shares NH 2 -terminal amino acid sequence with a newborn rat brain. The cDNA's of the bovine, human, and genes have been encode extraordinarily conserved proteins unrelated to known growth or neurotrophic factors, although identity nearly 50 percent has found predicted retinoic induced transcript in differentiating mouse embryonal carcinoma cells. Lysates COS-7 cells transiently expressing this were for NRK initiated neurite outgrowth mixed cultures embryonic brain RNA transcripts encoding widely distributed tissues developmentally regulated. This protein, previously designated as factor (HBGF)-8, is now renamed pleiotrophin (PTN) reflect its diverse activities. PTN may be first member family regulated cytokines.