The Yeast Cell Cycle Gene CDC34 Encodes a Ubiquitin-Conjugating Enzyme

• DOI: 10.1126/science.2842867
• Publication Date: 2006-10-05T21:21:15Z
• Authors: Mark G. Goebl; John Yochem; Stefan Jentsch; John P. McGrath; Alexander Varshavsky; Breck Byers
• Keywords: Ubiquitin-conjugating enzyme; SYT1; Deubiquitinating enzyme

Mutants in the gene CDC34 of yeast Saccharomyces cerevisiae are defective transition from G 1 to S phase cell cycle. This was cloned and shown encode a 295-residue protein that has substantial sequence similarity product RAD6 gene. The is required for variety cellular functions including DNA repair recently ubiquitin-conjugating enzyme. When produced Escherichia coli , catalyzed covalent attachment ubiquitin histones H2A H2B vitro, demonstrating another distinct member family enzymes. cycle function thus likely be mediated by activity its product.