Recognition of the Codon-Anticodon Helix by Ribosomal RNA

• DOI: 10.1126/science.285.5434.1722
• Publication Date: 2002-07-27T09:42:20Z
• Authors: Satoko Yoshizawa; Dominique Fourmy; Joseph D. Puglisi
• Keywords: Shine-Dalgarno sequence; Genetic Code; A-site; Translational frameshift; P-site

Translational fidelity is established by ribosomal recognition of the codon-anticodon interaction within aminoacyl–transfer RNA (tRNA) site (A site) ribosome. Experiments are presented that reveal possible contacts between 16 S and complex. N1 methylation adenine at position 1492 (A1492) A1493 interfered with A-site tRNA binding. Mutation A1492 to guanine or cytosine also impaired The deleterious effects A1492G A1493G (or both) mutations were compensated 2′fluorine substitutions in mRNA codon. results suggest ribosome recognizes complex messenger backbone provide a mechanism for molecular discrimination correct versus incorrect pairs.