Protein Interaction Mapping in C. elegans Using Proteins Involved in Vulval Development

• DOI: 10.1126/science.287.5450.116
• Publication Date: 2002-07-27T09:37:46Z
• Authors: Albertha J. M. Wa...; Raffaella Sordella; Xiaowei Lu; James L. Hartley; Gary F. Temple; Michael A. Brasch; Nicolas Thierry-Mieg; Marc Vidal
• Keywords: Model Organism

Protein interaction mapping using large-scale two-hybrid analysis has been proposed as a way to functionally annotate large numbers of uncharacterized proteins predicted by complete genome sequences. This approach was examined in Caenorhabditis elegans , starting with 27 involved vulval development. The resulting map reveals both known and new potential interactions provides functional annotation for approximately 100 gene products. A protein project is now feasible C. on genome-wide scale should contribute the understanding molecular mechanisms this organism human diseases.