Redox Signaling in Chloroplasts: Cleavage of Disulfides by an Iron-Sulfur Cluster

• DOI: 10.1126/science.287.5453.655
• Publication Date: 2002-07-27T09:40:21Z
• Authors: Shaodong Dai; Cristina Schwendt...; Peter Schürmann; S. Ramaswamy; Hans Eklund
• Keywords: Ferredoxin-thioredoxin reductase; Iron–sulfur cluster; Thiol

Light generates reducing equivalents in chloroplasts that are used not only for carbon reduction, but also the regulation of activity chloroplast enzymes by reduction regulatory disulfides via ferredoxin:thioredoxin reductase (FTR) system. FTR, key electron/thiol transducer enzyme this pathway, is unique it can reduce an iron-sulfur cluster, a property explained tight contact its active-site disulfide and center. The thin, flat FTR molecule makes two-electron possible forming on one side mixed with thioredoxin providing opposite access to ferredoxin delivering electrons.