Active Remodeling of Somatic Nuclei in Egg Cytoplasm by the Nucleosomal ATPase ISWI

• DOI: 10.1126/science.289.5488.2360
• Publication Date: 2002-07-27T09:48:21Z
• Authors: Nobuaki Kikyo; Paul A. Wade; Dmitry Guschin; Hui Ge; Alan P. Wolffe
• Keywords: Cloning (programming)

Cloning by the transplantation of somatic nuclei into unfertilized eggs requires a dramatic remodeling chromosomal architecture. Many proteins are specifically lost from nuclei, and others taken up egg cytoplasm. Recreating this exchange in vitro, we identified chromatin-remodeling nucleosomal adenosine triphosphatase (ATPase) ISWI as key molecule process. actively erases TATA binding protein association with nuclear matrix. Defining biochemistry global may facilitate efficiency cloning other dedifferentiation events that establish new stem cell lineages.