cDNA Expression Cloning of the IL-1 Receptor, a Member of the Immunoglobulin Superfamily

• DOI: 10.1126/science.2969618
• Publication Date: 2006-10-05T21:21:15Z
• Authors: John E. Sims; Caril J. March; David Cosman; Michael B. Widmer; H. Robson MacDonald; Catherine J. McMahan; Catherine E. Grubin; Janis M. Wignall; Jana L. Jackson; Susan M. Call; Della Friend; Alan R. Alpert; Steven Gillis; David L. Urdal; Steven K. Dower
• Keywords: Expression cloning; Interleukin-4 receptor; Immunoglobulin superfamily; Cloning (programming); Immunoglobulin domain

Interleukin-1 alpha and -1 beta (IL-1α IL-1β) are cytokines that participate in the regulation of immune responses, inflammatory reactions, hematopoiesis. A direct expression strategy was used to clone receptor for IL-1 from mouse T cells. The product cloned complementary DNA binds both IL-1α IL-1β a manner indistinguishable native cell receptor. extracellular, binding portion is 319 amino acids length composed three immunoglobulin-like domains. cytoplasmic 217 long.