Regulation of Class III Major Histocompatibility Complex Gene Products by Interleukin-1

• DOI: 10.1126/science.3010455
• Publication Date: 2006-10-05T20:28:52Z
• Authors: David H. Perlmutter; Gabriel Goldberger; Charles A. Dinarello; Steven B. Mizel; Harvey R. Colten
• Keywords: Complement factor B

Interleukin-1 (IL-1) is a product of mononuclear phagocytes that mediates changes characteristic the response to inflammation or tissue injury (the acute-phase response). One two structurally and functionally homologous major histocompatibility complex (MHC) class III genes encodes positive protein, complement factor B. The closely linked C2 gene not affected during response. Purified human IL-1, p H 7.0, recombinant-generated murine 5.0, increased expression B other proteins in hepatoma cells but decreased albumin, negative reactant. Furthermore, fibroblast L-cell line transfected with cosmid DNA bearing genes, IL-1 mediated reversible dose- time-dependent increase cells. Expression was by IL-1. effect on involves mechanism acting at pre-translational level as demonstrated an specific messenger RNA content corresponding biosynthesis secretion structural basis for selective independent regulation these provides insight into molecular control inflammatory