Subattomole Amino Acid Analysis by Capillary Zone Electrophoresis and Laser-Induced Fluorescence

• DOI: 10.1126/science.3140381
• Publication Date: 2006-10-05T21:21:15Z
• Authors: Yung-Fong Cheng; Norman J. Dovichi
• Keywords: Alanine; Fluorescein isothiocyanate

Subattomole analysis of fluorescein isothiocyanate (FITC) derivatives amino acids is accomplished by combining capillary zone electrophoresis for high-efficiency separation with laser-induced fluorescence high-sensitivity detection. Concentration detection limits range from 5 × 10 -12 molar alanine to 9 -11 lysine, injected in the column; -21 mole contained within ∼1-nanoliter injection volume at limit. The limit corresponds fewer than 6000 molecules onto column and represents an improvement four orders magnitude state art fluorescent six acids.