Cloning of Human Androgen Receptor Complementary DNA and Localization to the X Chromosome

• DOI: 10.1126/science.3353727
• Publication Date: 2006-10-05T21:21:15Z
• Authors: Dennis B. Lubahn; David R. Joseph; Patrick M. Sullivan; Huntington F. Wil...; Frank S. French; Elizabeth M. Wilson
• Keywords: 0301 basic medicine; X Chromosome; Base Sequence; Molecular Sequence Data; Chromosome Mapping; Cell Line; 03 medical and health sciences; Genes; Receptors, Androgen; Sequence Homology, Nucleic Acid; Animals; Humans; Amino Acid Sequence; Cloning, Molecular; Codon

The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using consensus nucleotide sequence DNA-binding domain family nuclear receptors. gene localized on between centromere and q13. Cloned complementary DNA, selected with an AR-specific oligonucleotide probe, expressed in monkey kidney (COS) cells yielded high-affinity androgen-binding protein steroid-binding specificity corresponding to that native AR. A predominant messenger RNA species 9.6 kilobases identified human, rat, mouse tissues known contain undetectable lacking activity, including liver androgen-insensitive mice. deduced amino acid within has highest identity progesterone receptor.