Cloning of Human Androgen Receptor Complementary DNA and Localization to the X Chromosome

0301 basic medicine 0303 health sciences X Chromosome Base Sequence Molecular Sequence Data Chromosome Mapping Cell Line 03 medical and health sciences Genes Receptors, Androgen Sequence Homology, Nucleic Acid Animals Humans Amino Acid Sequence Cloning, Molecular Codon
DOI: 10.1126/science.3353727 Publication Date: 2006-10-05T21:21:15Z
ABSTRACT
The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using consensus nucleotide sequence DNA-binding domain family nuclear receptors. gene localized on between centromere and q13. Cloned complementary DNA, selected with an AR-specific oligonucleotide probe, expressed in monkey kidney (COS) cells yielded high-affinity androgen-binding protein steroid-binding specificity corresponding to that native AR. A predominant messenger RNA species 9.6 kilobases identified human, rat, mouse tissues known contain undetectable lacking activity, including liver androgen-insensitive mice. deduced amino acid within has highest identity progesterone receptor.
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