Amitotic Neuroblastoma Cells Used for Neural Implants in Monkeys

• DOI: 10.1126/science.3749886
• Publication Date: 2006-10-05T20:28:52Z
• Authors: D. M. Gash; M. F. D. Notter; S. H. Okawara; A. L. Kraus; R. J. Joynt
• Keywords: Thymidine

The potential utility of cultured neuroblastoma cells as donor tissue for neutral implants into the mammalian brain has been examined. Cells from a human cell line, IMR-32, were labeled with [3H]thymidine and chemically rendered amitotic. These differentiated IMR-32 grafted hippocampi five adult African Green monkeys, graft survival was evaluated up to 270 days after transplantation. Autoradiographically identified in four animals. Processes could be followed distances 150 micrometers host brain. No evidence neoplastic growth transplant found. Thus, can survive prolonged periods primate may serve practical source neural implants.