Heterogeneous Nuclear Ribonucleoproteins: Role in RNA Splicing

• DOI: 10.1126/science.3952495
• Publication Date: 2006-10-05T20:28:52Z
• Authors: Yang Do Choi; Paula J. Grabowski; Phillip A. Sharp; Gideon Dreyfuss
• Keywords: Heterogeneous ribonucleoprotein particle; Spliceosome; Heterogeneous nuclear ribonucleoprotein; Precursor mRNA; Small nuclear ribonucleoprotein; Immunoprecipitation; Protein splicing

Splicing in vitro of a messenger RNA (mRNA) precursor (pre-mRNA) is inhibited by monoclonal antibody to the C proteins (anti-C) heterogeneous nuclear (hnRNA)-ribonucleoprotein (hnRNP) particles. This antibody, 4F4, inhibits an early step reaction: cleavage at 3′ end upstream exon and formation intron lariat. In contrast, boiled or different (designated 2B12) proteins, antibodies other hnRNP (120 68 kilodaltons) nonimmune mouse have no inhibitory effect. The 4F4 does not prevent adenosine triphosphate-dependent 60 S splicing complex (spliceosome). Furthermore, contains it can be immunoprecipitated with 4F4. Depletion from extract immunoadsorption either two (4F4 results loss activity, whereas mock-depletion has A form protein-depleted extract. These indicate essential role for mRNA precursors.