Myocardial Injury: Quantitation by Cell Sorting Initiated with Antimyosin Fluorescent Spheres

• DOI: 10.1126/science.7051286
• Publication Date: 2006-10-05T18:30:32Z
• Authors: B. A. Khaw; J. Scott; J. T. Fallon; S. L. Cahill; E. Haber; C. Homcy
• Keywords: Cell Sorting

Spheres coated with antibodies specific for myosin were used to detect myocardial cell membrane disruption by scanning electron microscopy. Injury in a population of cultured myocytes was then followed and measured fluorescence-activated sorting. This approach provides unique method quantitating the evolution injury potentially assessing efficacy interventions aimed at protection.