Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

• DOI: 10.1126/science.7542800
• Publication Date: 2006-10-27T18:19:42Z
• Authors: Robert D. Fleisch...; Mark D. Adams; Owen White; Rebecca A. Clayton; Ewen F. Kirkness; Anthony R. Kerlavage; Carol J. Bult; Jean-Francois Tomb; Brian A. Dougherty; Joseph M. Merrick; Keith McKenney; Granger Sutton; Will FitzHugh,; Chris Fields; Jeannine D. Gocayne; John Scott; Robert Shirley; Li-lng Liu; Anna Glodek; Jenny M. Kelley; Janice F. Weidman; Cheryl A. Phillips; Tracy Spriggs; Eva Hedblom; Matthew D. Cotton; Teresa R. Utterback; Michael C. Hanna; David T. Nguyen; Deborah M. Saudek; Rhonda C. Brandon; Leah D. Fine; Janice L. Fritchman; Joyce L. Fuhrmann; N. S. M. Geoghagen; Cheryl L. Gnehm; Lisa A. McDonald; Keith V. Small; Claire M. Fraser; Hamilton O. Smith; J. Craig Venter
• Keywords: Sequence assembly; Sequence (biology)

An approach for genome analysis based on sequencing and assembly of unselected pieces DNA from the whole chromosome has been applied to obtain complete nucleotide sequence (1,830,137 base pairs) bacterium Haemophilus influenzae Rd. This eliminates need initial mapping efforts is therefore applicable vast array microbial species which maps are unavailable. The H. Rd (Genome Sequence DataBase accession number L42023) represents only a free-living organism.