Restoration of T Cell Development in RAG-2-Deficient Mice by Functional TCR Transgenes

• DOI: 10.1126/science.8430336
• Publication Date: 2006-10-05T23:05:09Z
• Authors: Yoichi Shinkai; Shigeo Koyasu; Kei-ichi Nakayama; Kenneth M. Murphy; Dennis Y. Loh; Ellis L. Reinherz; Frederick W. Alt
• Keywords: Double negative; BETA (programming language)

Introduction of TCRα transgene, TCRβ or both into RAG-2 -/- mice differentially rescues T cell development. have small numbers TCR - CD4 CD8 (double negative, DN) thymocytes that express CD3γδε and ζ proteins intracellularly. a but not the background restored normal thymocytes. These cells were + positive, DP) expressed amounts surface chain dimers in association with ζ. α β transgenes developed DP single positive Thus, subunit, possibly novel CD3 complex, participates DN to transition.