RNA-Seq of single prostate CTCs implicates noncanonical Wnt signaling in antiandrogen resistance

• DOI: 10.1126/science.aab0917
• Publication Date: 2015-09-18T05:49:19Z
• Authors: David T. Miyamoto; Yu Zheng; Ben S. Wittner; Richard J. Lee; Huili Zhu; Katherine T. Brod...; Rushil Desai; Douglas B. Fox; Brian W. Brannigan; Julie Trautwein; Kshitij S. Arora; Niyati Desai; Douglas M. Dahl; Lecia V. Sequist; Matthew R. Smith; Ravi Kapur; Chin-Lee Wu; Toshi Shioda; Sridhar Ramaswamy; David T. Ting; Mehmet Toner; Shyamala Maheswaran; Daniel A. Haber
• Keywords: Male; 0301 basic medicine; Sequence Analysis, RNA; RNA Splicing; Prostate; Prostatic Neoplasms; Androgen Antagonists; Neoplastic Cells, Circulating; 3. Good health; Mice; 03 medical and health sciences; Drug Resistance, Neoplasm; Receptors, Androgen; Cell Line, Tumor; Proto-Oncogene Proteins; Benzamides; Nitriles; Phenylthiohydantoin; Animals; Humans; Single-Cell Analysis; Transcriptome; Signal Transduction

Circulating signals of drug resistance Cancer drugs often lose their effectiveness because tumors acquire genetic changes that confer resistance. Ideally, patients would be switched to a different before tumor growth resumes, but this requires early knowledge how arose. Miyamoto et al. have developed non-invasive method spot by sequencing RNA transcripts in single circulating cells (CTCs) (see the Perspective Nanus and Giannakakou). For example, prostate cancer patients, was triggered activation Wnt signaling pathway. But CTCs are rare fragile, technology needs further development it is used clinical practice. Science , issue p. 1351 ; see also 1283