Triggered recruitment of ESCRT machinery promotes endolysosomal repair

• DOI: 10.1126/science.aar5078
• Publication Date: 2018-04-05T20:58:44Z
• Authors: Michael L. Skowyra; Paul H. Schlesinger; Teresa V. Naismith; Phyllis I. Hanson
• Keywords: ESCRT

Endolysosomes can be damaged by diverse materials. Terminally compartments are degraded lysophagy, but pathways that repair salvageable organelles poorly understood. Here we found the endosomal sorting complex required for transport (ESCRT) machinery, known to mediate budding and fission on endolysosomes, also plays an essential role in their repair. ESCRTs were rapidly recruited acutely injured endolysosomes through a pathway requiring calcium ESCRT-activating factors was independent of lysophagy. We used live-cell imaging demonstrate responded small perforations endolysosomal membranes enabled recover from limited damage. Silica crystals disrupted triggered ESCRT recruitment. thus provide defense against damage likely relevant physiological pathological contexts.