Quantitative mass imaging of single biological macromolecules

• DOI: 10.1126/science.aar5839
• Publication Date: 2018-04-26T18:05:49Z
• Authors: Gavin Young; Nikolas Hundt; Daniel Cole; Adam Fineberg; Joanna Andrecka; Andrew Tyler; Anna Olerinyova; Ayla Ansari; Erik G. Marklund; Miranda P. Collier; Shane A. Chandler; Olga Tkachenko; Joel Allen; Max Crispin; Neil Billington; Yasuharu Takagi; James R. Sellers; Cédric Eichmann; Philipp Selenko; Lukas Frey; Roland Riek; Martin R. Galpin; Weston B. Struwe; Justin L. P. Benesch; Philipp Kukura
• Keywords: Macromolecular Substances

Watching proteins' weight Careful measurements of light scattering can provide information on individual macromolecules and complexes. Young et al. used a light-scattering approach for accurate mass determination proteins as small 20 kDa (see the Perspective by Lee Klenerman). Movies protein complex association dissociation were analyzed to extract biophysical parameters from single molecules assemblies without labeling. Using this approach, authors determined in vitro kinetics fibril aggregate growth constants protein-glycoprotein assembly. Science , issue p. 423 ; see also 378