Factors influencing meiotic recombination revealed by whole-genome sequencing of single sperm

• DOI: 10.1126/science.aau8861
• Publication Date: 2019-03-21T23:15:16Z
• Authors: Anjali Gupta Hinch; Gang Zhang; Philipp W. Becker; Daniela Moralli; Robert Hinch; Benjamin Davies; Rory Bowden; Peter Donnelly
• Keywords: Male; Pseudoautosomal Regions; 0301 basic medicine; Whole Genome Sequencing; Histone-Lysine N-Methyltransferase; Telomere; Spermatozoa; Chromatin; Mice, Inbred C57BL; Meiosis; Mice; 03 medical and health sciences; Animals; DNA Breaks, Double-Stranded; Crossing Over, Genetic; Single-Cell Analysis

Sequencing and the single sperm During meiosis, homologous chromosomes undergo doublestrand breaks in DNA that can cross over, shuffling genetic material. However, not every double-strand break resolves a crossover event. Hinch et al. wanted to determine rules governing recombination. They developed method sequence individual mouse applied it mice carrying two different alleles of protein involved mammalian crossovers. A high-resolution map revealed relationships between distribution crossovers, proteins recombination, specific factors determining whether becomes crossover. Science , this issue p. eaau8861