Structures and operating principles of the replisome

• DOI: 10.1126/science.aav7003
• Publication Date: 2019-01-25T00:17:07Z
• Authors: Yang Gao; Yanxiang Cui; Tara Fox; Shiqiang Lin; Huaibin Wang; Natalia de Val; Z. Hong Zhou; Wei Yang
• Keywords: Replisome

Structures of the simplest replisome The DNA performs concerted parental-strand separation and synthesis on both strands. Gao et al. report cryo–electron microscopy structures minimum set bacteriophage T7 proteins that can carry out leading- lagging-strand at replication fork (see Perspective by Li O'Donnell). Three key enzymes involved in replication—DNA polymerase, helicase, primase—were visualized complex with substrate DNA, demonstrating their highly dynamic organizations Comparison prokaryotic eukaryotic replisomes reveals evolutionarily conserved operating principles provides a structural basis for understanding coordination among replication, recombination, repair. Science , this issue p. eaav7003 ; see also 814