Single-cell nuclear architecture across cell types in the mouse brain

• DOI: 10.1126/science.abj1966
• Publication Date: 2021-09-30T19:07:36Z
• Authors: Yodai Takei; Shiwei Zheng; Jina Yun; Sheel Shah; Nico Pierson; Jonathan White; Simone Schindler; Carsten H. Tischb...; Guo-Cheng Yuan; Long Cai
• Keywords: Cell Nucleus; Cerebral Cortex; Neurons; 0301 basic medicine; 570; Genome; X Chromosome; Transcription, Genetic; 610; Endothelial Cells; Chromatin; Chromosomes; Epigenesis, Genetic; Mice; 03 medical and health sciences; Animals; Female; RNA-Seq; Single-Cell Analysis; Transcriptome; Neuroglia; In Situ Hybridization, Fluorescence

Nuclear organization in the brain

The brain consists of different cell types, neurons and glial cells, that have different nuclear architecture. Takei et al . used multiplexed imaging tools to examine the spatial arrangement of more than 3000 DNA loci, along with epigenetic marks and gene expression patterns, simultaneously in the same single cells in mouse brain cortex. They observed features that are conserved across cell types, as well as cell-type-dependent spatial arrangements at the megabase level. At the level of tens of kilobases, they observed similar single-cell chromosome domain conformations in both active and inactive X chromosomes that are averaged out in population-based measurements. —DJ