Fate mapping of neural stem cell niches reveals distinct origins of human cortical astrocytes

• DOI: 10.1126/science.abm5224
• Publication Date: 2022-05-19T17:59:39Z
• Authors: Denise E. Allen; Kevin C. Donohue; Cathryn R. Cadwell; David Shin; Matthew G. Keefe; Vikaas S. Sohal; Tomasz J. Nowakowski
• Keywords: Neocortex; Human brain; Progenitor; Cell fate determination; Fate mapping; Lineage (genetic)

Progenitors of the developing human neocortex reside in ventricular and outer subventricular zones (VZ OSVZ, respectively). However, whether cells derived from these niches have similar developmental fates is unknown. By performing fate mapping primary tissue, we demonstrate that astrocytes populate anatomically distinct layers. Cortical plate emerge VZ progenitors proliferate locally, while putative white matter are morphologically heterogeneous both OSVZ progenitors. Furthermore, via single-cell sequencing defined astrocyte subtypes using Patch-seq, identify molecular distinctions between VZ-derived cortical OSVZ-derived persist into adulthood. Together, our study highlights a complex role for cell lineage diversification neocortical astrocytes.