Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon

• DOI: 10.1126/science.adf3786
• Publication Date: 2023-10-12T18:00:31Z
• Authors: Nicola Micali; Shaojie Ma; Mingfeng Li; Suel-Kee Kim; Xoel Mato-Blanco; Suvimal Kumar Sindhu; Jon I. Arellano; Tianliuyun Gao; Mikihito Shibata; Kevin T. Gobeske; Alvaro Duque; Gabriel Santpere; Nenad Sestan; Pasko Rakic
• Keywords: Cell fate determination

During early telencephalic development, intricate processes of regional patterning and neural stem cell (NSC) fate specification take place. However, our understanding these in primates, including both conserved species-specific features, remains limited. Here, we profiled 761,529 single-cell transcriptomes from multiple regions the prenatal macaque telencephalon. We deciphered molecular programs organizing centers their cross-talk with NSCs, revealing primate-biased galanin-like peptide ( GALP ) signaling anteroventral Regional transcriptomic variations were observed along frontotemporal axis during stages neocortical NSC progression neurons astrocytes. Additionally, found that genes associated neuropsychiatric disorders brain cancer risk might play critical roles organizers progression.