Protein import into peroxisomes occurs through a nuclear pore–like phase

• DOI: 10.1126/science.adf3971
• Publication Date: 2022-12-15T18:57:24Z
• Authors: Yuan Gao; Michael L. Skowyra; Peiqiang Feng; Tom A. Rapoport
• Keywords: Peroxisomal targeting signal; Organelle; Nuclear pore

Peroxisomes are ubiquitous organelles whose dysfunction causes fatal human diseases. Most peroxisomal proteins imported from the cytosol in a folded state by soluble receptor PEX5. How cargo crosses membrane is unknown. Here, we show that import similar to nuclear transport. The protein PEX13 contains conserved tyrosine (Y)- and glycine (G)-rich YG domain, which forms selective phase resembling formed phenylalanine-glycine (FG) repeats within pores. resides two orientations oligomerize suspend meshwork lipid bilayer. Purified domains form hydrogels into PEX5 selectively partitions, using aromatic amino acid motifs, bringing along. thus an aqueous conduit through delivers peroxisomes.