SARS-CoV-2 spike protein–induced cell fusion activates the cGAS-STING pathway and the interferon response

• DOI: 10.1126/scisignal.abg8744
• Publication Date: 2022-04-12T17:59:09Z
• Authors: Xiaoman Liu; Liang Wei; Fengwen Xu; Fei Zhao; Yu Huang; Zhangling Fan; Shan Mei; Yamei Hu; Linxuan Zhai; Justin Guo; Aihua Zheng; Shan Cen; Chen Liang; Fei Guo
• Keywords: Cell Fusion; 0301 basic medicine; 03 medical and health sciences; SARS-CoV-2; Interferon Type I; Spike Glycoprotein, Coronavirus; COVID-19; Cytokines; Humans; Membrane Proteins; Nucleotidyltransferases; 3. Good health

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the unprecedented coronavirus disease 2019 (COVID-19) pandemic. Critical cases of COVID-19 are characterized by the production of excessive amounts of cytokines and extensive lung damage, which is partially caused by the fusion of SARS-CoV-2–infected pneumocytes. Here, we found that cell fusion caused by the SARS-CoV-2 spike (S) protein induced a type I interferon (IFN) response. This function of the S protein required its cleavage by proteases at the S1/S2 and the S2′ sites. We further showed that cell fusion damaged nuclei and resulted in the formation of micronuclei that were sensed by the cytosolic DNA sensor cGAS and led to the activation of its downstream effector STING. Phosphorylation of the transcriptional regulator IRF3 and the expression of IFNB , which encodes a type I IFN, were abrogated in cGAS-deficient fused cells. Moreover, infection with VSV-SARS-CoV-2 also induced cell fusion, DNA damage, and cGAS-STING–dependent expression of IFNB . Together, these results uncover a pathway underlying the IFN response to SARS-CoV-2 infection. Our data suggest a mechanism by which fused pneumocytes in the lungs of patients with COVID-19 may enhance the production of IFNs and other cytokines, thus exacerbating disease severity.