The actin-binding protein CAP1 represses MRTF-SRF–dependent gene expression in mouse cerebral cortex

• DOI: 10.1126/scisignal.adj0032
• Publication Date: 2024-05-07T17:58:29Z
• Authors: Sharof Khudayberdiev; Kerstin Weiss; Anika Heinze; Dalila Colombaretti; Nathan Trausch; Uwe Linne; Marco B. Rust
• Keywords: Myocardin; Actin-binding protein

Serum response factor (SRF) is an essential transcription for brain development and function. Here, we explored how SRF cofactor, the actin monomer-sensing myocardin-related MRTF, regulated in mouse cortical neurons. We found that MRTF-dependent activity vitro vivo was repressed by cyclase-associated protein CAP1. Inactivation of actin-binding CAP1 reduced amount monomers cytoplasm, which promoted nuclear MRTF translocation MRTF-SRF activation. This function independent cofilin1 actin-depolymerizing factor, loss neurons not compensated endogenous CAP2. Transcriptomic proteomic analyses cerebral cortex lysates from wild-type