Measurement and Clinical Monitoring of Human Lymphocyte Clonality by Massively Parallel V-D-J Pyrosequencing

• DOI: 10.1126/scitranslmed.3000540
• Publication Date: 2009-12-29T23:04:08Z
• Authors: Scott D. Boyd; Eleanor L. Marshall; Jason D. Merker; Jay M. Maniar; Lyndon N. Zhang; Bita Sahaf; Carol D. Jones; Birgitte B. Simen; Bozena Hanczaruk; Khoa D. Nguyen; Kari C. Nadeau; Michael Egholm; David B. Miklos; James L. Zehnder; Andrew Z. Fire
• Keywords: Adult; 0301 basic medicine; B-Lymphocytes; Neoplasm, Residual; Genes, Immunoglobulin Heavy Chain; Sequence Analysis, DNA; Middle Aged; Clone Cells; High-Throughput Screening Assays; Gene Expression Regulation, Neoplastic; Young Adult; 03 medical and health sciences; Treatment Outcome; Reference Values; Case-Control Studies; Hematologic Neoplasms; Humans; Receptors, Immunologic; Gene Rearrangement, B-Lymphocyte; Aged; DNA Primers; Gene Library

Massively parallel sequencing of rearranged immune receptor genes permits detection and tracking of specific immune cell populations in normal and pathological contexts.