Two Human Host Defense Ribonucleases against Mycobacteria, the Eosinophil Cationic Protein (RNase 3) and RNase 7

• DOI: 10.1128/aac.00428-13
• Publication Date: 2013-05-29T04:58:59Z
• Authors: David Pulido; Marc Torrent; David Andreu; M. Victoria Nogués; Ester Boix
• Keywords: Antimycobacterial

ABSTRACT There is an urgent need to develop new agents against mycobacterial infections, such as tuberculosis and other respiratory tract or skin affections. In this study, we have tested two human antimicrobial RNases mycobacteria. RNase 3, also called the eosinophil cationic protein, 7 are small proteins secreted by innate cells during host defense. Both induced upon infection displaying a wide range of antipathogen activities. particular, they released leukocytes epithelial cells, contributing tissue protection. Here, been proven effective Mycobacterium vaccae at low micromolar level. High bactericidal activity correlated with their bacterial membrane depolarization permeabilization Further analysis on both protein-derived peptides identified for 3 N-terminus fragment that even more active than parental protein. Also, potent agglutinating was unique its derived peptide. The particular biophysical properties peptide envisaged suitable reference development novel antimycobacterial drugs. results support contribution immune response