Efflux Pumps, OprD Porin, AmpC β-Lactamase, and Multiresistance in Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients

• DOI: 10.1128/aac.00816-09
• Publication Date: 2010-03-02T04:54:26Z
• Authors: Maria Tomás; Michel Doumith; Marina Warner; Jane F. Turton; Alejandro Beceiro; German Bou; David M. Livermore; Neil Woodford
• Keywords: 0301 basic medicine; Cystic Fibrosis; Reverse Transcriptase Polymerase Chain Reaction; 610; Membrane Transport Proteins; Porins; Drug Resistance, Multiple; beta-Lactamases; Electrophoresis, Gel, Pulsed-Field; Up-Regulation; 03 medical and health sciences; Bacterial Proteins; 616; Drug Resistance, Bacterial; Mutation; Pseudomonas aeruginosa; Humans; Pseudomonas Infections; Bacterial Outer Membrane Proteins

ABSTRACT

Expression of ampC , oprD , mexA , mexC , mexE , and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with β-lactam resistance. A specific combination of mexR , nalC , and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA .