Transcriptional Profiling of Azole-Resistant Candida parapsilosis Strains

• DOI: 10.1128/aac.01127-10
• Publication Date: 2011-04-26T03:04:49Z
• Authors: A. P. Silva; I. M. Miranda; A. Guida; J. Synnott; R. Rocha; R. Silva; A. Amorim; C. Pina-Vaz; G. Butler; A. G. Rodrigues
• Keywords: Posaconazole; Azole; Efflux; Candida parapsilosis; Ergosterol

ABSTRACT Herein we describe the changes in gene expression profile of Candida parapsilosis associated with acquisition experimentally induced resistance to azole antifungal drugs. Three resistant strains C. were obtained following prolonged vitro exposure a susceptible clinical isolate constant concentrations fluconazole, voriconazole, or posaconazole. We found that after incubation fluconazole became both azoles but not posaconazole, although susceptibility this decreased, whereas strain incubated posaconazole displayed three azoles. The and voriconazole have increased transcription factor MRR1 , major facilitator transporter MDR1 several reductases oxidoreductases. Interestingly, similarly what has been described albicans upregulation is correlated point mutations strains. shows two factors ( UPC2 NDT80 ) 13 genes involved ergosterol biosynthesis. This first study addressing global molecular mechanisms underlying ; results suggest tolerance involves activation efflux pumps and/or synthesis.