Effects of Troponoids on Mitochondrial Function and Cytotoxicity

• DOI: 10.1128/aac.01617-21
• Publication Date: 2021-10-25T18:50:23Z
• Authors: Daniel P. Bradley; Austin T. O’Dea; Molly E. Woodson; Qilan Li; Nathan L. Ponzar; Alaina Knier; Bruce L. Rogers; Ryan P. Murelli; John E. Tavis
• Keywords: 0301 basic medicine; Hepatitis B virus; 03 medical and health sciences; Ribonuclease H; Humans; Reactive Oxygen Species; Virus Replication; Tropolone; Mitochondria; 3. Good health

The α-hydroxytropolones (αHTs) are troponoid inhibitors of hepatitis B virus (HBV) replication that can target HBV RNase H with submicromolar efficacies. αHTs and related troponoids (tropones and tropolones) can be cytotoxic in cell lines as measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H -tetrazolium (MTS) assays that assess mitochondrial function. Previous studies suggest that tropolones induce cytotoxicity through inhibition of mitochondrial respiration.