Impact of Hydroxychloroquine-Loaded Polyurethane Intravaginal Rings on Lactobacilli

• DOI: 10.1128/aac.01819-15
• Publication Date: 2015-09-29T02:38:25Z
• Authors: Yannick Leandre T...; Yufei Chen; Anne-Marie Bernier; Emmanuel A. Ho
• Keywords: Hydroxychloroquine; Viability assay; Vaginal flora; Vaginal microbicide

The use of polymeric devices for controlled sustained delivery drugs is a promising approach the prevention HIV-1 infection. Unfortunately, certain microbicides, when topically applied vaginally, may be cytotoxic to vaginal epithelial cells and protective microflora present within female genital tract. In this study, we evaluated impact hydroxychloroquine (HCQ)-loaded, reservoir-type, polyurethane intravaginal rings (IVRs) on growth Lactobacillus crispatus jensenii viability ectocervical cells. IVRs were fabricated using hot-melt injection molding capable providing release HCQ 24 days, with mean daily rates 17.01 ± 3.6 μg/ml in sodium acetate buffer (pH 4) 29.45 4.84 MRS broth 6.2). Drug-free released had no significant effects bacterial or Furthermore, there was integrity cell monolayers, comparison controls, as measured by transepithelial electrical resistance. Overall, first study evaluate HCQ-loaded flora monolayers.