Assessment of Clofazimine and TB47 Combination Activity against Mycobacterium abscessus Using a Bioluminescent Approach

• DOI: 10.1128/aac.01881-19
• Publication Date: 2019-12-16T13:00:25Z
• Authors: Yang Liu; Yaoju Tan; M. Mahmudul Islam; Yuanyuan Cao; Xiaoyun Lu; Sheng Zeng; H. M. Adnan Hameed; Peipei Zhou; Xingshan Cai; Shuai Wang; Julius N. Mugweru; Guoliang Zhang; Huancai Yin; Jianxiong Liu; Eric Nuermberger; Tianyu Zhang
• Keywords: Mice, Inbred BALB C; 0303 health sciences; Luminescence; Mycobacterium abscessus; Mycobacterium Infections, Nontuberculous; Drug Synergism; Microbial Sensitivity Tests; Clofazimine; Anti-Bacterial Agents; 3. Good health; Electron Transport; Drug Combinations; Electron Transport Complex III; Mice; 03 medical and health sciences; Bacterial Proteins; Clarithromycin; Drug Resistance, Multiple, Bacterial; Animals; Female; Enzyme Inhibitors; Genetic Engineering; Amikacin

Mycobacterium abscessus is intrinsically resistant to most antimicrobial agents. The emerging infections caused by M. abscessus and the lack of effective treatment call for rapid attention. Here, we intended to construct a selectable marker-free autoluminescent M. abscessus strain (designated UAlMab) as a real-time reporter strain to facilitate the discovery of effective drugs and regimens for treating M. abscessus . The UAlMab strain was constructed using the dif /Xer recombinase system.