Bactericidal and Fungicidal Activity of N -Chlorotaurine Is Enhanced in Cystic Fibrosis Sputum Medium

• DOI: 10.1128/aac.02527-16
• Publication Date: 2017-02-22T01:30:22Z
• Authors: Martina Gruber; Ivan Moser; Markus Nagl; Michaela Lackner
• Keywords: Aspergillus terreus

ABSTRACT Lung infections with multiresistant pathogens are a major problem among patients suffering from cystic fibrosis (CF). N -Chlorotaurine (NCT), microbicidal active chlorine compound no development of resistance, is well tolerated upon inhalation. The aim this study was to investigate the in vitro bactericidal and fungicidal activity NCT artificial sputum medium (ASM), which mimics composition CF mucus. inoculated bacteria ( Staphylococcus aureus , including some methicillin-resistant S. [MRSA] strains, Pseudomonas aeruginosa Escherichia coli ) or spores fungi Aspergillus fumigatus terreus Candida albicans Scedosporium apiospermum boydii Lomentospora prolificans aurantiacum minutisporum Exophiala dermatitidis Geotrichum sp.), final concentrations 10 7 8 CFU/ml. added at 37°C, time-kill assays were performed. At concentration 1% (10 mg/ml, 55 mM) NCT, killed within min 15 min, respectively, detection limit 2 CFU/ml (reduction 5 6 log units). Reductions units still achieved 0.1% (bacteria) 0.3% (fungi) largely 30 min. Measurements by means iodometric titration showed oxidizing for 1, 30, 60, >60 0.1%, 0.3%, 0.5%, 1.0% matches killing test results. demonstrated broad-spectrum milieu mucus ideal clinical use. ASM even stronger than that buffer solution; particularly pronounced fungi. This finding can be explained formation, through transhalogenation, monochloramine, rapidly penetrates pathogens.