Tromantadine: inhibitor of early and late events in herpes simplex virus replication

• DOI: 10.1128/aac.22.6.1031
• Publication Date: 2012-06-28T00:03:40Z
• Authors: K S Rosenthal; M S Sokol; R L Ingram; R Subramanian; R C Fort
• Keywords: Vero cell; Cytopathic effect; Viral culture; Simplexvirus

Unlike amantadine (1-adamantanamine), tromantadine (N-1-adamantyl-N-[2-(dimethyl amino)ethoxy]acetamide hydrochloride) inhibits herpes simplex virus type 1 (KOS strain)-induced cytopathic effect and replication with limited toxicity to the cells. Vero HEp-2 cells tolerated up 2 mg of per X 10(6) for 24-, 48-, or 96-h incubation periods little change in cell morphology. Treatment 10 50 micrograms reduced virus-induced effect. 100 500 inhibited production. Complete inhibition production was observed treatments mg. The antiherpetic activity dependent upon viral inoculum size time addition compound respect infection. Virion synthesis polypeptide were by at infection 4 h postinfection. results are consistent an early event infection, before macromolecular synthesis, a late event, such as assembly release virus.