Effect of an arginine-glycine-aspartic acid-containing peptide on hematogenous candidal infections in rabbits

• DOI: 10.1128/aac.36.1.132
• Publication Date: 2012-06-28T00:05:50Z
• Authors: S A Klotz; R L Smith; B W Stewart
• Keywords: Ex vivo; Aspartic acid

The adherence of Candida albicans yeast cells to the subendothelial extracellular matrix, fibronectin, laminin, and type I IV collagen was tested. Fibronectin (10(-7) M) a peptide, PepTite-2000 (Telios Pharmaceuticals Inc., San Diego, Calif.), containing sequence arginine-glycine-aspartic acid (RGD) inhibited these targets by greater than 90%. When C. perfused over ex vivo rabbit aortic endothelium, there no significant difference in amount presence or absence RGD-containing peptide. However, peptide reduced number organisms present liver, brain, heart, kidneys (P less 0.05) rabbits 4 h after intravenous inoculation 5 x 10(7) cells. also macroscopic abscesses 72 0.05). Inhibition vitro may occur because blocks fungal receptor that is necessary for adherence.