Genetic basis for clarithromycin resistance among isolates of Mycobacterium chelonae and Mycobacterium abscessus

• DOI: 10.1128/aac.40.7.1676
• Publication Date: 2018-10-09T00:25:57Z
• Authors: R J Wallace; A Meier; B A Brown; Y Zhang; P Sander; G O Onyi; E C Böttger
• Keywords: 23S ribosomal RNA; Mycobacterium abscessus; Mycobacterium chelonae

Resistance to clarithromycin among isolates of Mycobacterium chelonae and M. abscessus was observed in 18 800 (2.3%) patients tested between 1990 1995. Patients whose were resistant had either disseminated disease or chronic lung disease, the recovered after monotherapy. Sequencing gene coding for 23S rRNA peptidyltransferase region revealed a point mutation involving adenine at position 2058 (38%) 2059 (62%) 20 relapse from first 13 identified. By pulsed-field gel electrophoresis random amplified polymorphic DNA PCR, initial shown have identical patterns. found only single chromosomal copy operon, thus making them susceptible single-step mutations. Thus, resistance these species rapidly growing mycobacteria relates occurs limited clinical situations, but identified almost 5%