Fungicidal action of aureobasidin A, a cyclic depsipeptide antifungal antibiotic, against Saccharomyces cerevisiae

• DOI: 10.1128/aac.41.3.672
• Publication Date: 2018-10-09T00:28:15Z
• Authors: M Endo; K Takesako; I Kato; H Yamaguchi
• Keywords: Depsipeptide; Mechanism of Action

Aureobasidin A, an antifungal antibiotic inhibiting a wide range of pathogenic fungi, is lethal for growing cells susceptible fungi. We did cytological studies on the mechanism its fungicidal action against Saccharomyces cerevisiae. When cultures were treated with 5.0 micrograms aureobasidin A per ml, numbers viable started to decrease after 2 3 h incubation, and most had lost viability 5 6 h. cell death in began, amino acids released by could be detected, indicating disruption membrane. The proportion single small bud or two more buds increased as population decreased. Most such DNA content G2 phase cycle, suggesting that drug inhibited some cellular process involved normal growth but not affect replication. Disruption actin assembly was found many h, chitin delocalization. results suggest has previously unknown toward S. It causes aberrant assembly, budding leading death, probably through destruction membrane integrity.