Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa

• DOI: 10.1128/aac.41.7.1601
• Publication Date: 2018-10-09T00:28:57Z
• Authors: W H Haas; K Schilke; J Brand; B Amthor; K Weyer; P B Fourie; G Bretzel; V Sticht-Groh; H J Bremer
• Keywords: 0303 health sciences; 03 medical and health sciences; Genes, Bacterial; Africa; Molecular Sequence Data; Point Mutation; Mycobacterium tuberculosis; Catalase; Peroxidase; 3. Good health

A sample of 124 isoniazid (INH)-resistant and 88 susceptible strains Mycobacterium tuberculosis complex from south, central, west Africa was analyzed by direct sequence analysis PCR-restriction fragment length polymorphism their catalase-peroxidase (katG) genes. Point mutations at codon 315 were found in the genomes 64% INH-resistant strains, but no complete deletions identified. Mutations 463 independent INH resistance linked to geographic origins strains.