Influence of the TonB Energy-Coupling Protein on Efflux-Mediated Multidrug Resistance inPseudomonas aeruginosa

• DOI: 10.1128/aac.42.9.2225
• Publication Date: 2018-10-09T00:33:24Z
• Authors: Qixun Zhao; Xian-Zhi Li; Anita Mistry; Ramakrishnan Srik...; Li Zhang; Olga Lomovskaya; Keith Poole
• Keywords: Efflux

ABSTRACT TonB couples the energized state of cytoplasmic membrane to operation outer receptors responsible for Fe(III) siderophore uptake across gram-negative bacteria. A tonB mutant Pseudomonas aeruginosa deficient in iron was shown present study be hypersusceptible a wide variety antibiotics, reminiscent phenotype mutants defective mexAB-oprM antibiotic efflux operon. This not related influences mutation on status cell, and indeed, intrinsic susceptibility expression were unaffected by levels growth medium. The presence multicopy plasmid increased level resistance MexAB-OprM + strain but that − antimicrobial agents. not, however, altered deletion mutant, indicating any influence MexAB-OprM-mediated multidrug at pump activity. Consistent with this, drug accumulation assays revealed exhibited decreased efflux. Still, nalB wholly abrogated mutation, it is likely an essential component apparatus. Similarly, elimination from nfxB only partially compromised MexCD-OprJ-mediated resistance. Intriguingly, following , suggesting may also mediated determinants other than (and MexCD-OprJ). Thus, plays important role both acquired P. .