Characterization of Plasmids Carrying CMY-2 from Expanded-Spectrum Cephalosporin-Resistant Salmonella Strains Isolated in the United States between 1996 and 1998

• DOI: 10.1128/aac.46.5.1269-1272.2002
• Publication Date: 2002-07-27T10:09:01Z
• Authors: A. Carattoli; F. Tosini; W. P. Giles; M. E. Rupp; S. H. Hinrichs; F. J. Angulo; T. J. Barrett; P. D. Fey
• Keywords: Salmonella typhimurium; 0301 basic medicine; Cephalosporin Resistance; Ceftriaxone; bacterial DNA; ceftriaxone; cephalosporin; gene product; protein cmy2; unclassified drug, antibiotic resistance; article; bacterium conjugation; bacterium isolation; DNA determination; hybridization; nonhuman; nucleotide sequence; plasmid; priority journal; Salmonella; United States, beta-Lactamases; Ceftriaxone; Cephalosporin Resistance; Cephalosporins; Conjugation, Genetic; Drug Resistance, Multiple, Bacterial; Humans; Microbial Sensitivity Tests; Plasmids; Salmonella; Salmonella Infections; Salmonella typhimurium; United States; Microbial Sensitivity Tests; United States; beta-Lactamases; Cephalosporins; 3. Good health; 03 medical and health sciences; Salmonella; Conjugation, Genetic; Drug Resistance, Multiple, Bacterial; Salmonella Infections; Humans; Plasmids

ABSTRACT

Sequencing of DNA from 15 expanded-spectrum cephalosporin (e.g., ceftriaxone)-resistant Salmonella isolates obtained in the United States revealed that resistance to ceftriaxone in all isolates was mediated by cmy-2. Hybridization patterns revealed three plasmid structures containing cmy-2 in these 15 isolates. These data suggest that the spread of cmy-2 among Salmonella strains is occurring through mobilization of the cmy-2 gene into different plasmid backbones and consequent horizontal transfer by conjugation.