Fengycins, Cyclic Lipopeptides from Marine Bacillus subtilis Strains, Kill the Plant-Pathogenic Fungus Magnaporthe grisea by Inducing Reactive Oxygen Species Production and Chromatin Condensation

• DOI: 10.1128/aem.00445-18
• Publication Date: 2018-07-04T11:51:45Z
• Authors: Linlin Zhang; Chaomin Sun
• Keywords: Magnaporthe; Lipopeptide

Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult control. Bacillus species have been extensively explored for biological control of many fungal diseases. In present study, marine bacterium subtilis BS155 showed strong antifungal activity against M. The active metabolites were isolated identified as cyclic lipopeptides (CLPs) fengycin family, named BS155, combination high-performance liquid chromatography (HPLC) electrospray ionization mass spectrometry (ESI-MS) tandem (ESI-MS/MS). Analyses using scanning transmission electron microscopy revealed that morphological changes in plasma membrane cell wall hyphae. Using comparative proteomic biochemical assays, was demonstrated reduce mitochondrial potential (MMP), induce bursts reactive oxygen (ROS), downregulate expression level ROS-scavenging enzymes. Simultaneously, chromatin condensation hyphal cells, which led upregulation DNA repair-related protein cleavage poly(ADP-ribose) polymerase (PARP). Altogether, our results indicate acts inducing damage dysfunction organelles, disrupting MMP, oxidative stress, condensation, resulting death. Therefore, its parent are very promising candidates associated rice should be further investigated such.IMPORTANCE (Oryza sativa L.) most important crop primary source more than half world's population. Notably, scientists China developed several types can grown seawater, avoiding use precious freshwater resources potentially creating enough 200 million people. plant-affecting fungus causal agent disease, rather chemical this threatening disease highly desirable. work, we discovered lipopeptide material produced Our elucidate mechanism BS155-mediated growth inhibition highlight B. biocontrol cultivation under both fresh- saltwater conditions.