Global Distribution Patterns and Pangenomic Diversity of the Candidate Phylum “Latescibacteria” (WS3)

• DOI: 10.1128/aem.00521-17
• Publication Date: 2017-03-18T03:01:17Z
• Authors: Ibrahim F. Farag; Noha H. Youssef; Mostafa S. Elshahed
• Keywords: DNA, Bacterial; 0301 basic medicine; Bacteria; Genetic Variation; Fresh Water; Genomics; 15. Life on land; Cellulosomes; 03 medical and health sciences; Bacterial Proteins; RNA, Ribosomal, 16S; 14. Life underwater; Groundwater; Ecosystem; Phylogeny

We investigated the global distribution patterns and pangenomic diversity of candidate phylum "Latescibacteria" (WS3) in 16S rRNA gene as well metagenomic data sets. document distinct for various orders sets, with prevalence sediment_1 terrestrial, PBSIII_9 groundwater temperate freshwater, GN03 pelagic marine, saline-hypersaline, wastewater habitats. Using a fragment recruitment approach, we identified 68.9 Mb "Latescibacteria"-affiliated contigs publicly available sets comprising 73,079 proteins. Metabolic reconstruction suggests prevalent saprophytic lifestyle all orders, marked capacities degradation proteins, lipids, polysaccharides predominant plant, bacterial, fungal/crustacean, eukaryotic algal cell walls. As well, extensive transport central metabolic pathways metabolism imported monomers were identified. Interestingly, genes domains suggestive production cellulosome-e.g., protein-coding harboring dockerin I attached to glycosyl hydrolase scaffoldin-encoding cohesin CBM37 domains-were order PBSIII_9, GN03, MSB-4E2 fragments recovered from four anoxic aquatic habitats; hence extending cellulosomal capabilities Bacteria beyond Gram-positive Firmicutes In addition fermentative pathways, complete electron chain terminal cytochrome c oxidases Caa3 (for operation under high oxygen tension) Cbb3 low oxygenated partially/seasonally Our effort represents comprehensive view this yet-uncultured provides insights broader than complementary those gained genome recovery initiatives focusing on single or few sampled environments.IMPORTANCE understanding phylogenetic diversity, capabilities, ecological roles microorganisms is rapidly expanding. However, recent efforts mainly have been focused recovering genomes novel microbial lineages specific sampling site, rather wide range environmental To comprehensively evaluate genomic landscape, putative phyla, that focus habitats adequately sample intraphylum within target lineage are needed. Here, results preference habitats, plant polysaccharide abilities occurrence genes/domains necessary cellulosomes three (GN03, MSB-4E2) anaerobic locations, identification components an aerobic respiratory chain, multiple O2-dependent reactions The demonstrate value phylocentric surveys panmetabolic since they provide more single-cell and/or metagenomic-enabled site.