Characterization of the Genome of the Dairy Lactobacillus helveticus Bacteriophage ΦAQ113

• DOI: 10.1128/aem.00620-13
• Publication Date: 2013-06-01T05:15:48Z
• Authors: Miriam Zago; Erika Scaltriti; Lia Rossetti; Alessandro Guffanti; Angelarita Armiento; Maria Emanuela Fo...; Stefano Grolli; Domenico Carminati; Elena Brini; Paolo Pavan; Armando Felsani; Annalisa D'Urzo; Anna Moles; Jean-Baptiste Claude; Rita Grandori; Roberto Ramoni; Giorgio Giraffa
• Keywords: Lactobacillus helveticus; Temperateness; Lysogenic cycle; Lactobacillus gasseri; ORFS; Phagemid; Lytic cycle

ABSTRACT The complete genomic sequence of the dairy Lactobacillus helveticus bacteriophage ΦAQ113 was determined. Phage is a Myoviridae with an isometric capsid and contractile tail. final assembled consensus revealed linear, circularly permuted, double-stranded DNA genome size 36,566 bp G+C content 37%. Fifty-six open reading frames (ORFs) were predicted, putative function assigned to approximately 90% them. shows functionally related genes clustered together in structure composed modules for replication/regulation, packaging, head tail morphogenesis, cell lysis, lysogeny. identification involved establishment lysogeny indicates that it may have originated as temperate phage, even if isolated from natural cheese whey starters virulent because able propagate sensitive host strain. Additionally, we discovered phage closely gasseri KC5a johnsonii Lj771 genomes. phylogenetic similarities between L. two phages belong gut species confirm possible common ancestral origin support increasing consideration health-promoting organism.