Tetracycline induces wsp operon expression to promote biofilm formation in Pseudomonas putida

• DOI: 10.1128/aem.01071-24
• Publication Date: 2024-11-26T14:00:20Z
• Authors: Kexin Mu; Meina He; Haozhe Chen; Tong Liu; Ying Fan; Yongxin Tao; Haoqi Feng; Qiaoyun Huang; Yujie Xiao; Wenli Chen
• Keywords: Pseudomonas putida; Pseudomonadales

The overuse and wanton discharge of antibiotics produces a threat to bacteria in the environment, which, turn, stimulates more rapid emergence antibiotic-resistant bacteria. Pseudomonas putida actively forms biofilms protect population under tetracycline stress, but molecular mechanism remains unclear. This study found that at sub-minimal inhibitory concentrations increased cyclic diguanylate (c-di-GMP), second messenger positively regulates biofilm formation. Four c-di-GMP-metabolizing proteins were be involved tetracycline-mediated promotion, including DibA, WspR, PP_3242, PP_3319. Among them, cyclase WspR displayed most significant effect on c-di-GMP level belongs wsp operon comprising seven genes (wspA-wspF wspR). contained six promoters, one major start promoter (PwspA) five internal promoters (PwspB, PwspC, PwspD, PwspF, PwspR), promoted activity PwspA. stress-response sigma factor RpoS directly bound PwspA regulated its stress. Moreover, was required for induce promote Our results enrich transcriptional regulation reveal by which promotes formation P. putida.IMPORTANCEThe against antibiotic threats, Here, our showed treatment could expression Wsp system via putida, resulting elevated levels, leads contains binds activity.