Current Production and Metal Oxide Reduction by Shewanella oneidensis MR-1 Wild Type and Mutants

• DOI: 10.1128/aem.01087-07
• Publication Date: 2007-07-20T20:52:51Z
• Authors: Orianna Bretschger; Anna Obraztsova; Carter A. Sturm; In Seop Chang; Yuri A. Gorby; Samantha B. Reed; David E. Culley; Catherine L. Reardon; Soumitra Barua; Margaret F. Romine; Jizhong Zhou; Alexander S. Beliaev; Rachida Bouhenni; Daad Saffarini; Florian Mansfeld; Byung-Hong Kim; James K. Fredrickson; Kenneth H. Nealson
• Keywords: 0301 basic medicine; Shewanella; 0303 health sciences; Oxides; Gene Expression Regulation, Bacterial; Ferric Compounds; Electron Transport; 03 medical and health sciences; Manganese Compounds; Genes, Bacterial; Mutation; Oxidation-Reduction

ABSTRACT

Shewanella oneidensis MR-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. S. oneidensis MR-1 can reduce Mn(IV) and Fe(III) oxides and can produce current in microbial fuel cells. The mechanisms that are employed by S. oneidensis MR-1 to execute these processes have not yet been fully elucidated. Several different S. oneidensis MR-1 deletion mutants were generated and tested for current production and metal oxide reduction. The results showed that a few key cytochromes play a role in all of the processes but that their degrees of participation in each process are very different. Overall, these data suggest a very complex picture of electron transfer to solid and soluble substrates by S. oneidensis MR-1.